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. 2016 Jan 8;6:19083. doi: 10.1038/srep19083

Figure 6. Study design.

Figure 6

In phase 1 (3-to 21- weeks of age) and phase 2 (22-to 60- weeks of age) of the study, C57BL/6N female mice were fed control (CON, 10% kcal from fat), a high fat (HF, 45% kcal from fat), or a calorie restricted diet (ER, 30% caloric restricted compared to the control), leading to 3 × 3 groups of intervention at the endpoint (control- control (C-C), control-high fat (C-H), control-energy restriction (C-E), high fat-control (H-C), high fat- high fat (H-H), high fat-energy restriction (H-E), energy restriction-control (E-C), energy restriction-high fat (E-H), and energy restriction-energy restriction (E-E)). From 16- to 21- weeks of age, all mice received a weekly intraperitoneal injection of AOM at a dose of 10 mg/kg for 6 weeks. All mice were euthanized at 60 weeks of age and the colonic mucosal microbiota samples were collected in one representative animal in each cage at 60 weeks of age.