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. 2016 Jan 8;6:19073. doi: 10.1038/srep19073

Figure 3. Expression of osteogenic and adipogenic genes in ADSCs infected with Ad-Runx2.

Figure 3

(a) Runx2 mRNA was detected by real-time RT-PCR in ADSCs infected with Ad-EGFP or Ad-Runx2 at 1, 3, 7, 10 and 14 days post-infection. (b–d) The expression of specific osteogenic genes, including OCN (b), BSP (c) and COLI (d) in ADSCs infected with Ad-EGFP or Ad-Runx2 at 1, 3, 7, 10 and 14 days post-infection, as assayed by real-time PCR. (e,f) The expressions of the specific adipogenic markers LPL (e) and PPARγ (f) mRNA levels by real-time RT-PCR in ADSCs infected with Ad-EGFP or Ad-Runx2 at 1, 3, 7, 10 and 14 days post-infection. (g) Analysis of ALP activity in the lysates of ADSCs infected with Ad-Runx2 or Ad-EGFP in at 2 3, 7, 10 and 14 days post-infection. ALP activity in Runx2-infected cells was elevated at day 3, continued to increase with a peak at 10 days, and then gradually declined, but remained at a high level for the duration of the experiment. The intrinsic cellular ALP activity in Ad-EGFP-infected cells remained unchanged. The experiments were performed three times, and representative images are shown. Values are mean ± SEM, **P < 0.01, *P < 0.05 vs. Ad-EGFP-ADSCs.