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. 2016 Jan 8;6:19073. doi: 10.1038/srep19073

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Copyright © 2016, Macmillan Publishers Limited

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(a–d) 12 weeks after surgery, the CT images were acquired to measure the CSA (cross sectional area) of the bone tunnels. First, we located the bone tunnel in cross sections (a). Then, using CT 3D reconstruction, the corresponding sagittal (b) and coronal (c) images were obtained to ensure that the orientation was perpendicular to the long axis of the bone tunnel. Based on the orientation, a series of cross sections were created, resulting in 1.0 mm-thick slices with no interslice gap (d). (e,f) Average diameters of bone tunnels (e) and the ossification areas (f) were analyzed from the CT 3D reconstruction of the joint after ACL reconstruction surgery treated with Ad-Runx2-ADSCs, fibrin glue or RNAi Runx2. Values are mean ± SEM, n = 10, *P < 0.05, **P < 0.01 as indicated.