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. 2016 Jan 8;6:19073. doi: 10.1038/srep19073

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Gross observation demonstrated no significant adverse effect of the treatments with Ad-Runx2-ADSCs, fibrin glue or RNAi Runx2 on the joint tissue. Note the lack of ossification or scar abundance at any of the intra-articular tissues, such as ligaments or menisci. (b) Histological photomicrographs of specimens post-surgery. Specimens were harvested and observed 2 (i, ii, iii), 4 (iv, v, vi), 6 (vii, viii, ix), 12 (x, xi, xii), 26 (xiii, xiv, xv), and 52 weeks (xvi, xvii, xviii) after surgery. Specimens were divided into three groups, includingAd-Runx2-ADSCs treated (i, iv, vii, x, xiii, xvi); fibrin glue treated (ii, v, viii, xi, xiv, xvii); and RNAi Runx2-treated (iii, vi, ix, xii, xv, xviii). T, tendon; IF, interface tissue; FC, fibrous cartilage; B, bone; CC, calcified cartilage; S, Sharpey fiber. Representative images from four replications are shown.