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. 2016 Jan 8;6:19074. doi: 10.1038/srep19074

Figure 7. DHA inhibits the proliferation, migration, and invasion of MDA-MB-231 cells and promotes apoptosis.

Figure 7

(A) Viability of DHA-treated MDA-MB-231 cells. The calculated IC50 was 74.26 μM (48 h) and 53.4 μM (96 h) respectively. (B) DHA inhibits monolayer wound healing of MDA-MB-231 cells. (C) Membrane-associated, crystal violet stained MDA-MB-231 breast cancer cells following treatment with various concentrations of DHA for 24 h. (D) The areas of invasive cells were counted. (E) Flow cytometric analysis of DHA-treated MDA-MB-231 cells. (F) DHA reduces phosphorylated AKT expression, but does not effect the expression of SRC. (G) The band intensity corresponding to phosphorylated AKT was quantified and normalized to GAPDH using Image J. (*p < 0.05, **p < 0.01).