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. 2016 Jan 8;6:19106. doi: 10.1038/srep19106

Figure 7. Cultured hippocampal neurons from offspring of polyI:C injected mothers exhibit larger amplitude and shorter rise time of mIPSCs.

Figure 7

(A) Representative voltage-clamp recording of mIPSCs in cultured hippocampal neurons from offspring of saline (top) and polyI:C (bottom)-injected mothers. (B) Cumulative probability-amplitude plots show larger amplitude of mIPSCs in cultured hippocampal neurons from offspring and polyI:C-injected mothers compared to that from offspring of saline-treated moms (I = 43.4 ± 0.5 pA, n = 28 cells from 6 polyI:C-injected mothers versus I = 38.6 ± 0.5 pA, n = 30 cells from 6 saline-injected mothers; p < 0.0001). (C) Cumulative probability-interevent interval plots show no differences in the mIPSC frequency of cultured hippocampal neurons from offspring of saline and polyI:C-injected mothers (n = 27 cells and n = 24 cells, respectively, in each group from 6 different mothers). (D) no differences were found in the mIPSC decay time in cultured hippocampal neurons from offspring of saline and polyI:C-injected mothers (n = 30 cells and n = 28 cells, respectively, in each group from 6 different mothers). (E) Cultured hippocampal neurons from offspring of polyI:C injected mothers exhibit shorter mean rise time of mIPSCs (3.4 ± 0.06 ms, n = 28 cells from 6 polyI:C-injected mothers versus 3.8 ± 0.06 ms ,n = 30 cells from 6 saline-injected mothers; t(2733) = −4.88, p < 0.0001). For each recorded neuron, 50 events were analyzed.