Table 1. Intrinsic excitability parameters of cultured hippocampal neurons from offspring of saline and polyI:C-injected moms.
| Resting potential (mV) | Input resistancea (MΩ) | Threshold potentialb (mV) | AP amplitude (mV) | AP width at threshold (ms) | Rheobasec (pA) | |
|---|---|---|---|---|---|---|
| Saline-injected mothers | −59.3 ± 1.2 (n = 44) | 236 ± 8.8 (n = 41) | −33.6 ± 0.6 (n = 37) | 81.2 ± 1.7 (n = 44) | 2.40 ± 0.15 (n = 44) | 409 ± 19 (n = 44) |
| PolyI:C injected mothers | −60.5 ± 1.0 (n = 54) | 200.5 ± 8.5 ** (n = 56) | −34.5 ± 0.6 (n = 55) | 87.2 ± 1.7 * (n = 54) | 2.10 ± 0.08 * (n = 54) | 594 ± 25 ** (n = 54) |
The parameters were measured in numbers of neurons (n) from offspring taken from 6 different mothers (N). The membrane potential values were corrected for the liquid junction potential (5.0 mV).
* and ** indicates statistical significance of p < 0.05 and p < 0.01, respectively (assessed by two-sample assuming equal variances t-test).
aCalculated from the voltage change following a −300 pA current injection for 800 ms (Rin = V/I in MΩ).
bMeasured as the potential at the beginning of the AP upstroke.
cThe rheobase current was determined by injecting neurons with a series of current steps starting from 200 pA, followed by 50 pA increments steps for a 2 ms pulse duration, until a single action potential (AP) was elicited.