Figure 3.

Effect of Ncl on salt tolerance in terms of leave SPAD value (a), shoot dry weight (b), and ion (Na⁺, K⁺, and Cl⁻ leaf contents (c) in BC₄F₃ lines developed by MAS by introducing the tolerance allele from a wild soybean accession JWS156-1 into Jackson. BC₄F₃-J1T and BC₄F₃-J1S was derived from progeny of self-pollination of a BC₄F₂ plant, which was heterozygous in the Ncl region. (d) Performance of BC₄F₃-J1T and BC₄F₃-J1S after treatment with 100 mM NaCl for approximately three weeks in a hydroponic condition. Data are shown as mean ± s.d. (n = 11). **indicate significant difference at P < 0.01 levels versus Jackson (Dunnett’s multiple comparison test).