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. 2016 Jan 8;6:19111. doi: 10.1038/srep19111

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Copyright © 2016, Macmillan Publishers Limited

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(a) Schematic summary of cell therapy using day20 CMs. (b) Engraftment ratio after injection of day20 iPSC-CMs based on luminescence imaging data (n = 16). Values are mean ± SE. (c) Representative images of luminescence signals after injection into the hearts of NOG mice with myocardial infarction. (d) Upper: Immunostaining of engrafted CMs in the whole heart for cTNT (red), anti-luc (yellow), and Hoechst (blue). Scale bar: 300 μm. Images were photographed by a fluorescence microscope, BZ-X700 (Keyence). Lower: Trichrome-staining of the additional section of the upper image. (e) Upper: Magnified image of (d). Red, cTNT; yellow, anti-luc; blue, Hoechst. Scale bar: 100 μm. Lower: Trichrome-staining of the additional section of the upper image. (f) Immunostaining of engrafted CMs at high magnification. Red, cTNI; yellow, human nuclei; blue, Hoechst. Scale bar: 20 μm.