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. 2016 Jan 8;6:19111. doi: 10.1038/srep19111

Figure 5. Follow-up analysis showed efficient engraftment over a long time and maturation of engrafted CMs in vivo in infarcted hearts.

Figure 5

(a) Engraftment ratio over 6 months after injection of day20 CMs based on luminescence imaging data (day0–3 months: n = 16; 3 months-6 months: n = 5). Values are mean ± SE. (b) The luminescence signal ratio at several time points. The ratio was defined as the luminescence signal intensity at the end of the time interval divided by the intensity at the beginning of the interval. Values are mean ± SE.(c) Immunostaining of engrafted CMs in the whole heart at 6 months: red, cTNT; yellow, anti-luc; blue, Hoechst. Scale bar: 300 μm. Images were photographed by a fluorescence microscope BZ-X700 (Keyence). (d) Magnified image of (c): red, cTNT; yellow, anti-luc; blue, Hoechst. Scale bar: 100 μm. (e) Immunostaining of engrafted CMs at high magnification: red, cTNI; yellow, human nuclei; blue, Hoechst. Scale bar: 20 μm. (f) Transmission electron micrographs of iPSC-CMs at 2 months and 6 months after transplantation. Z, Z-disk; M, M-band; I, I-band; H, H-band; A, A-band; Mit, Mitochondrion; FA, Fascia Adherens; Ds, Desmosome; Gap, Gap junction. Scale bars: 500 nm.