Table 1.

Overall quantitative characterization of blood clot contraction in different experimental conditions

Compound added to whole blood	Degree of contraction, %	Lag time, s	AUC, a.u.	Average velocity, %/s (×10−3)
Calcium chloride (n = 25)
0 (control)	54 ± 1	78 ± 11	762 ± 15	43 ± 1
2 mM	53 ± 1	76 ± 9	772 ± 9	42 ± 1
5 mM	52 ± 1	67 ± 8	79 ± 14	41 ± 1
10 mM	49 ± 2*	65 ± 9	797 ± 15	39 ± 1*
Thrombin (n = 11)
0.5 U/mL	32 ± 3	89 ± 16	948 ± 21	25 ± 2
1 U/mL	50 ± 2***	79 ± 16	832 ± 23***	40 ± 1***
Blebbistatin (n = 3)
0 (control)	40 ± 2	155 ± 28	890 ± 25	33 ± 2
25 µM	28 ± 3**	165 ± 22	994 ± 21*	23 ± 2**
50 µM	25 ± 2***	169 ± 24	1054 ± 41***	20 ± 1***
100 µM	18 ± 2****	185 ± 50	1056 ± 17***	15 ± 2****
200 µM	15 ± 1****	218 ± 45	1088 ± 9****	12 ± 1****
RGDS peptide (n = 8)
0 (control)	43 ± 1	111 ± 7	913 ± 18	36 ± 2
0.5 mM	24 ± 2***	251 ± 45	1020 ± 23***	23 ± 3**
1 mM	24 ± 1***	332 ± 55*	1061 ± 13***	21 ± 3***
2 mM	22 ± 1***	273 ± 59	1067 ± 12***	17 ± 1***
Iodoacetamide (n = 5)
0 (control)	49 ± 3	75 ± 22	820 ± 30	38 ± 2
1 mM	33 ± 1**	78 ± 27	950 ± 20*	26 ± 1***
Cystamine (n = 4)
0 (control)	53 ± 3	71 ± 22	793 ± 66	43 ± 3
0.1 mM	48 ± 3	101 ± 17	836 ± 44	40 ± 2
0.5 mM	43 ± 3	139 ± 28	909 ± 29	35 ± 2
1 mM	38 ± 5	161 ± 65	931 ± 27	31 ± 4
2 mM	36 ± 5*	124 ± 62	960 ± 20	28 ± 4*

Mean ± standard error of the mean (SEM). *P < .05 when compared with control; **P < .01 when compared with control; ***P < .001 when compared with control or 0.5 U/mL for thrombin; ****P < .0001 when compared with control.