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. 2015 Nov 17;44(1):315–329. doi: 10.1093/nar/gkv1260

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — DDK phosphorylation of Mcm2 is required for Cdc45 recruitment to Mcm2–7 by Sld3. mcm2-td cells expressing wild-type mcm2, mcm2–2A and mcm2–2D were grown as described in Material and Methods. (A, left panel) Whole cell extracts were analyzed by western blot for the expression of the indicated proteins. (A, right panel) Cells were immunoprecipitated with antibodies directed against Mcm2, followed by western analysis with antibodies directed against Mcm2, Mcm10, Sld3 and Cdc45. (B) Results from experiments similar to those shown in (A) were quantified, averaged and plotted. Graphs represent mean values from two independent experiments and error bars indicate the standard deviation of the mean.