Figure 2.

Sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of recombinant wild-type (WT) and mutant VSV L proteins. The WT and mutant VSV L proteins (1 μg) were analyzed by 7.5% SDS-PAGE followed by Coomassie Brilliant Blue staining. M lanes show marker proteins with the indicated molecular masses. The names of the point mutants contain the original amino acid (one-letter code) at the indicated position in the VSV L protein followed by the replacement amino acid. The HR-RH mutant carrying the H1227R and R1228H mutations (lane 33) is a representative of the previously identified cap-defective mutants (16).