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. 2015 Nov 23;44(1):330–341. doi: 10.1093/nar/gkv1286

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Cap-defective mutant L proteins are not able to synthesize full-length mRNAs efficiently. The WT and mutant VSV L proteins (0.15 μg) were subjected to in vitro transcription reactions with [α-³²P]GTP, the other three NTPs, the recombinant P protein (0.05 μg) and the native N-RNA complex (0.4 μg). After treatment with RNase H and oligo(dT), transcripts were analyzed by 20% (A) or 5% (B) urea-PAGE followed by autoradiography. Lanes 1, 8, 20 and 28 indicate no L protein. M lanes show marker RNAs with the indicated lengths. The positions of previously identified transcripts (18) are indicated on the right. Le indicates the leader RNA.