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. 2015 Nov 23;44(1):330–341. doi: 10.1093/nar/gkv1286

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Cap-defective mutant L proteins produce uncapped abortive short transcripts. Transcripts, synthesized by the WT or cap-defective mutant L proteins, were post-labeled by vaccinia virus capping enzyme in the presence of [α-³²P]GTP and analyzed by 20% urea-PAGE followed by autoradiography. Lanes 1 and 4 indicate no L protein. Lane 11 indicates a longer exposure of lane 7. The positions of previously identified capped RNAs (18) are shown.