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. 2015 Nov 23;44(1):330–341. doi: 10.1093/nar/gkv1286

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — The cap-defective mutant L proteins synthesize small amounts of polyadenylated full-length (N₁) and 5′-truncated (N₂) N mRNA. ³²P-Labeled transcripts, synthesized by the WT or mutant L protein, were treated with or without RNase H and oligo(dT) and analyzed by 5% urea-PAGE followed by autoradiography. The sample volume of transcripts synthesized with the WT L protein (lanes 1, 2, 5 and 6) was 20-fold smaller than those of transcripts synthesized with the other mutants.