Table 1. Relative enzymatic activities of mutant L proteins.
| Mutant | Relative activity (% of WT)a | ||||
|---|---|---|---|---|---|
| PRNTase | GTPase | RdRp | |||
| GpppA | L-pRNA | Pi | Leader RNA | mRNAs | |
| W1094A | 136 ± 5 | 96 ± 4 | 86 ± 6 | 33 ± 6 | 17 ± 3 |
| W1094F | 181 ± 10 | 239 ± 32 | 102 ± 11 | 98 ± 8 | 88 ± 17 |
| G1100A | 11 ± 1 | 10 ± 3 | 99 ± 12 | 128 ± 11 | 6 ± 2 |
| P1104A | 12 ± 1 | 13 ± 2 | 242 ± 26 | 27 ± 3 | 14 ± 1 |
| P1104V | 15 ± 0 | 10 ± 1 | 124 ± 17 | 84 ± 8 | 39 ± 4 |
| Y1152A | 0 ± 0 | 3 ± 3 | 42 ± 4 | 0 ± 0 | 0 ± 0 |
| Y1152F | 4 ± 0 | 9 ± 9 | 161 ± 20 | 49 ± 3 | 30 ± 0 |
| Y1152W | 0 ± 0 | 3 ± 2 | 42 ± 6 | 1 ± 0 | 0 ± 0 |
| G1154A | 0 ± 0 | 3 ± 3 | 35 ± 1 | 0 ± 1 | 0 ± 0 |
| S1155A | 20 ± 1 | 20 ± 1 | 90 ± 4 | 56 ± 8 | 34 ± 4 |
| S1155T | 44 ± 4 | 40 ± 4 | 142 ± 9 | 30 ± 3 | 20 ± 7 |
| S1155G | 53 ± 3 | 68 ± 3 | 244 ± 11 | 38 ± 6 | 30 ± 6 |
| S1155V | 2 ± 0 | 3 ± 3 | 187 ± 15 | 4 ± 0 | 0 ± 0 |
| T1157A | 0 ± 0 | 1 ± 1 | 127 ± 1 | 80 ± 7 | 2 ± 0 |
| T1157S | 0 ± 0 | 1 ± 1 | 159 ± 1 | 66 ± 5 | 3 ± 0 |
| L1153A | 10 ± 5 | 12 ± 3 | 83 ± 4 | 0 ± 2 | 0 ± 0 |
| L1153V | 39 ± 3 | 48 ± 2 | 124 ± 17 | 3 ± 1 | 3 ± 1 |
| L1153I | 45 ± 15 | 98 ± 1 | 187 ± 23 | 9 ± 3 | 15 ± 3 |
| L1153F | 3 ± 0 | 5 ± 2 | 96 ± 14 | 1 ± 1 | 3 ± 2 |
| K1156A | 81 ± 6 | 104 ± 1 | 187 ± 26 | 109 ± 11 | 122 ± 24 |
| S1158A | 88 ± 10 | 87 ± 1 | 111 ± 26 | 119 ± 17 | 48 ± 10 |
| W1188A | 0 ± 0 | 1 ± 1 | 102 ± 3 | 4 ± 1 | 0 ± 0 |
| W1188F | 0 ± 0 | 2 ± 2 | 16 ± 2 | 13 ± 2 | 1 ± 0 |
| F1269A | 0 ± 0 | 3 ± 3 | 163 ± 5 | 38 ± 2 | 3 ± 0 |
| F1269Y | 31 ± 6 | 35 ± 8 | 107 ± 7 | 80 ± 6 | 85 ± 4 |
| F1269W | 18 ± 2 | 21 ± 0 | 130 ± 5 | 114 ± 2 | 37 ± 4 |
| Q1270A | 0 ± 0 | 2 ± 2 | 108 ± 11 | 102 ± 4 | 5 ± 0 |
| Q1270N | 0 ± 0 | 1 ± 1 | 162 ± 19 | 16 ± 1 | 1 ± 0 |
| HR-RH | 0 ± 0 | 3 ± 3 | 92 ± 5 | 101 ± 7 | 4 ± 0 |
aThe PRNTase, GTPase and RdRp activities of the mutant L proteins were measured by the in vitro oligo-RNA capping, L-pRNA intermediate formation, GTPase and transcription assays as shown in Figures 3 and 4. Relative enzymatic activities of the mutant L proteins were expressed as percentages of the WT activities. Data represent the means and standard deviations from three independent experiments.