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. 2015 Nov 24;44(1):342–353. doi: 10.1093/nar/gkv1306

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Primer extension analysis of m¹A1408 modification by W203 substituted CacKam proteins. (A) Autoradiogram of RT primer extension of 16S rRNA from 30S subunits treated with W203A or W203F substituted CacKam protein reveals strong m¹A1408 methylation for the latter enzyme only (indicated by strong termination at nucleotide C1409, indicated with an arrow). (B) Representative comparison of RT primer extension following wild-type CacKam and CacKam-W203F modification of 30S using an increasing enzyme to substrate ratio. (C) Quantification of RT analyses from panel B.