Table 2. Impact on CacKam activity of substitutions of residues predicted to mediate interaction with 30S.

Protein region	Amino acid substitution	Kanamycin MIC (μg/ml)
N-terminus	K13A	512
	K14A	1024
β1/2 linker	R43A	16
β2/3 linker	R73A	16
	R76A	256
	K77A	16
	K80A	16
β5/6 linker	R151A	1024
	R159A	>1024
β6/7 linker	R189A	>1024
	R205A	256
	R206A	16