Table 3. Summary of RNA-seq and qRT-PCR results for the 16 genes selected for verification.
| Gene | RNA-seq FC | Inconsistency index (I) | qRT-PCR FC | n (pcmWT1,pcm14) | P-value (t-test) | pre-mRNA FC | n (pcmWT1,pcm14) | P-value (t-test) |
|---|---|---|---|---|---|---|---|---|
| CG16712 | +inf | 0.61 | 130.8 | 6,6 | 0.0027 | |||
| CG4716 | +inf | 0.55 | 102.2 | 6,6 | 0.0004 | |||
| CecC | +inf | 0.36 | 616.5 | 6,5 | 0.0012 | 103.1 | 5,5 | 0.0093 |
| CG13403 | +inf | 0.26 | 6.0 | 6,6 | 0.0156 | |||
| AttA | +inf | 0.68 | 580.6 | 6,6 | 0.0003 | |||
| CG14292 | +inf | 0.71 | 1.2 | 6,6 | 0.8936 | |||
| dilp8 | 650.1 | 0.49 | 6600.0 | 6,5 | <0.0001 | 10.3 | 5,5 | <0.0001 |
| Lsp1β | 62.1 | 0.80 | 32.8 | 6,5 | 0.056 | |||
| Corin | 51.8 | 0.40 | 1.4 | 6,5 | 0.3128 | 61.7 | 5,5 | 0.0001 |
| CG1208 | 39.7 | 0.58 | 4.9 | 6,6 | 0.0282 | |||
| Nplp2 | 26.8 | 0.47 | 38.2 | 6,6 | 0.0002 | 1.7 | 5,5 | 0.4925 |
| Ets21C | 23.6 | 0.40 | 70.3 | 6,6 | <0.0001 | 29.5 | 5,5 | <0.0001 |
| gd | 22.5 | 0.36 | 18.1 | 6,5 | <0.0001 | |||
| CG1909 | 19.3 | 0.38 | 7.2 | 6,6 | <0.0001 | |||
| cac | 18.9 | 0.38 | 10.4 | 6,5 | <0.0001 | |||
| Clect27 | 8.5 | 0.12 | 10.0 | 6,6 | 0.0002 | |||
| rpr | 4.3 | 0.24 | 7.6* | 6,6 | <0.0001 | 1.7* | 6,6 | 0.081 |
*indicates data from (15). P-values presented are from individual t-tests comparing pacman null transcript levels to wild-type and have not been adjusted for multiple comparisons.