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. 2015 Dec 10;44(1):294–303. doi: 10.1093/nar/gkv1379

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 1. — Mistranslation in A. pernix occurs during low-temperature growth. (A) tRNA microarray showing high-fidelity tRNA charging with Met during growth at 90°C. (B) tRNA microarray showing tRNA^Leu misacylation with Met during growth at 75°C. (C) Array map showing locations of relevant tRNA probes. (D) Quantification of tRNA^Leu misacylation from panel B as a percent of tRNA^Met signal. (E) tRNA microarray excluding the possibility of Met-tRNA cross-hybridization to Leu probes through addition of free Met probes in hybridization mixture. (F) Quantification of Met blocked panel E and unblocked panel B showing the intensity of tRNA^Leu signal relative to tRNA^Met signal. (G) tRNA microarray excluding the possibility of signal emanating from tRNA thiomodifications by chemical deacylation of charged tRNA prior to array hybridization.