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. 2015 Sep 8;44(1):117–133. doi: 10.1093/nar/gkv885

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 1. — Screening for Polycomb group proteins (PcGs) regulated by E2F1. (A) Gene expression heatmap of representative E2F1 targets and PcG genes that are differentially regulated in SK-Mel-147 upon E2F1 knockdown (11). Red and green shading indicates high and low gene expression relative to sh.control set to 1. (B and C) Analysis of PcGs at mRNA (B) and protein levels (C) following knockdown of E2F1 in SK-Mel-147 cell line. E2F1 target gene BIRC5 is shown as positive control. ß-actin served as loading control. (D and E) mRNA (D) and protein levels (E) of EPC1 and EZH2 in indicated cell lines after induction of ER.E2F1. Saos-2 or SK-Mel-29 cells stable expressing ER-E2F1 were treated with 4-hydroxytamoxifen (4-OHT, 1 μM) or solvent for 24 hrs. Actin was used for loading control. (F) Expression of EPC1 (green) and E2F1 (red) in SK-Mel-29.ERE2F1 treated with 4-OHT for 24 h. Nuclei were counter-stained with DAPI (blue) and fluorescence was visualized by laser scanning microscopy.