Figure 8.

EPC1-A and EPC1-B domains alone are sufficient to modulate transcriptional activity of E2F1. (A and B) H1299 cells were co-transfected with expression plasmids for full-length EPC1, Flag-tagged EPC1-A or EPC1-B together with Bcl-2-luc (A) or p27-luc promoter (B) reporter constructs in the absence or presence of transient E2F1 expression. Expression of truncated EPC1 proteins was confirmed by Western blot by using anti-FLAG antibody. Actin was used as loading control. Promoter assays were performed 24 h post transfection using pcDNA plasmid as control that was set to 1. Data are presented as the mean with standard deviation of technical duplicates in an experiment representative of several independent others. Statistical significance was determined by two-sided Student's t-test. *P ≤ 0.05, **P ≤ 0.01.