Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Sep 10;44(1):134–151. doi: 10.1093/nar/gkv889

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 9. — Effect of cAMP on in vitro binding of Cmr to clustered binding sites adjacent to genes belonging to DosR regulon. Schematic maps (not drawn to scale) and EMSA showing multiple Cmr binding sites in the (A) Rv2030c, (B) Rv2623 and (C) Rv1738. Cmr binding sites (red boxes), DosR binding sites (green boxes), and probes used in EMSA (blue lines with Cmr binding sites) are shown. A total of 1 μM Cmr and 1 mM cAMP was used in this set of EMSA experiments, wherever indicated. About 500× cold DNA identical to probe was used as specific competition (S). About 500× annealed oligos from an unrelated region (listed in Supplementary Table S3) was used as non-specific competition (NS).