Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Sep 17;44(1):175–186. doi: 10.1093/nar/gkv928

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 2. — Igκ locus contraction in pro-B and pre-B cells is independent of iEκ. (A) Schematic representation of the murine Igκ locus including the bacterial artificial chromosome clones used as 3D FISH probes: distal Vκ – Alexa488, proximal Vκ – Cy5, Cκ – Alexa568. The indicated distances separating each of the 3 probes and their positions within the Igκ locus were determined from the Ensembl mouse genome database. (B) Representative images of the Igκ locus in the 3 B-cell subsets. (C) Scatter plots show the distances between distal Vκ, proximal Vκ, and Cκ regions in cultured E2A^−/− pre-pro-B, Rag1^−/− pro-B, Rag1^−/− Igμ pre-B and Rag2^−/− κE1/E2^mut pre-B cells. For each condition 2–3 mice were used and at least 200 alleles were analyzed per population. Red horizontal lines represent median distances. The non-parametric Mann-Whitney test was used to calculate significance levels: *, P < .05; ***, P < .001; ****, P < .0001. (D) Cumulative frequency plots showing the distribution of spatial distances between the distal Vκ, proximal Vκ and Cκ region probes in the 4 progenitor-B cell subsets.