Figure 6.

Positioning away from the nuclear lamina increases germline transcription of Igh and Igκ. (A) Representative microscope images indicate differential nuclear positioning of Igh (red) and Igκ (green) loci in E2A^−/− pre-pro-B-cells cultured with (+) or without (−) TSA. Bar graphs showing the frequencies of (B) Ch regions and (C) Cκ regions positioned within a distance range from the nuclear lamina in E2A^−/− pre-pro-B cultured with (+) or without (−) TSA. >100 alleles were acquired for each condition. Germline transcript levels of (D) iEμ and (E) iEκ as assessed with RQ-PCR in 2 independent cultures. Bars represent average with SD. ND, not detectable. Dashed lines indicate the detection limit at cycle-threshold 40.