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. 2015 Sep 29;44(1):198–209. doi: 10.1093/nar/gkv957

Figure 5.

Figure 5.

KAT5-dependent activation of ATM by FA. (A) ATM acetylation in A549 treated with 300 μM FA for 3 h. Immunoprecipitated ATM was probed with anti-acetyl-lysine (acK) antibodies. (B) Absence of ATM acetylation in FA-treated cells with inactive KAT5. Cells were treated with FA in the absence or presence of KAT5i (25 μM NU9056 added 1 h before FA) and analyzed for ATM acetylation as in panel A. (C) Suppression of ATM activation by KAT5 inhibitor (KAT5i—25 μM NU9056, added for 1 h before FA) in WI38 and (D) IMR90 cells. WI38 cells were treated with FA for 1 h and IMR90 for 3 h. (E) Loss of ATM signaling in IMR90 cells with KAT5 knockdown by siRNA. Cells were treated with FA for 3 h. Images for si-Control and si-KAT5 are from the same blot after removal of intervening bands. (F) Suppression of ATM autophosphorylation by c-ABL inhibition. IMR90 cells were treated for 3 h with imatinib before addition of FA for 3 h.