Fig. 5.

Changes in isoform phosphorylation of the target signaling protein, AKT, in response to ischemia. Representative spectra of time-dependent changes in the phospho3 isoform phosphorylation of AKT between N0 and N45 in normal tissue are shown in a and b. An overview of all isoforms of AKT in normal tissue of all patients analyzed is shown in c; therein results were displayed as fold changes normalized in normal tissue to sample NC5/N0 and in tumor tissue to sample NC5/T0. The same graphs are shown for time-dependent changes between T0 and T20 in the phospho5 isoform in tumor tissue (d, e). An overview of all isoforms of AKT in tumor tissue of all patients analyzed is shown in f. Kruskal–Wallis test and Dunn test for multiple comparisons or analysis of variance (ANOVA) were used for statistical analysis. N normal tissue, T tumor tissue, 0 before surgery, 10, 20, 45 10, 20, 45 min after resection; Time points (10, 20, 45) are compared with time point 0; ^a p ≤ 0.05; ^b p ≤ 0.01; ^c p ≤ 0.001