Figure 1.

Concordant depletion of myelin-associated glycoprotein (MAG) levels with reduction of axonal filament medium chain (neurofilament (NF160)) in relapsing experimental autoimmune encephalomyelitis (EAE) (B6 x SJL) F1 mice.

Upper – Profound depletion of MAG levels in relapsing H-2^b/s mice. Dissimilar regulation of relative levels of MAG was observed between H-2^b and H-2^b/s mice throughout EAE. Levels of MAG in spinal cord of EAE mice were measured by western blot. Representative blot of 3–4 similar experiments shows: acute – H-2^b/s (13 dpi, grade 4.5), H-2^b (16 dpi, grade 4); relapse – H-2^b/s (21 dpi, grade 3.5, 1^st relapse), H-2^b (27 dpi, grade 2, 1st relapse); and chronic-sustained – H-2^b/s (72 dpi, grade 2.5). Compared to levels in control and in H-2^b mice, H-2^b/s mice markedly reduced their relative levels of MAG during acute and chronic period. Most profound depletion of MAG was found in relapsing H-2^b/s mice.

Lower – Distinctive reduction of NF160 levels in central nervous system in relapsing H-2^b/s compared to H-2^b mice. Relative levels of medium chain of axonal filament (NF160) were significantly depleted in mice with relapsing and chronic disease. Relative levels of NF160 were measured similarly as levels of MAG (upper). Most profound drop in NF160 levels was observed in relapsing H-2^b/s mice.

*Reference to Figure from Skundric et al. (2008).