TABLE 1.
Strains, plasmids, and primers used in this study
| Strain, plasmid, or primer | Description or sequence | Reference or source |
|---|---|---|
| Strains | ||
| 0100993 | Capsular serotype 3 clinical isolate | 12 |
| phgA mutant | 0100993 containing an insertion in phgA (Sp1043); Cmr | This study |
| phgC mutant | 0100993 containing an insertion in phgC (Sp1045); Cmr | This study |
| PPC50 | 0100993 containing an insertion downstream of phgC; Cmr | This study |
| Plasmids | ||
| pID701 | Disruption vector for S. pneumoniae derived from pEVP3; Cmr | 12 |
| pPC33 | pID701 with ORF3.1/ORF3.2 PCR product ligated into the XbaI site; Cmr | This study |
| pPC49 | pID701 with ORF5.6/ORF5.7 PCR product ligated into the XbaI site; Cmr | This study |
| pPC50 | pID701 with ORF5.8/ORF5.9 PCR product ligated into the XbaI site; Cmr | This study |
| Primers (5′ to 3′) | ||
| ORF3.1 | GCT CTA GAG TAA ATT ACC AAG TGA GG | |
| ORF3.2 | CGC TCT AGA TCA CCT GTA TAG GGT CG | |
| ORF5.6 | GCT CTA GAA CCC TAC TTC TGG TGG C | |
| ORF5.7 | CGC TCT AGA TTG TGA ATC GCC TCA GGC | |
| ORF5.8 | GCT CTA GAT CGG TTC TCT GCC TGA GGC | |
| ORF5.9 | CGC TCT AGA TTT GCA AGG AAT GTC CGT TG | |
| O2.1 | GAA GTG AAG ACG GTG AAG CG | |
| O3.3 | CCT TGT AAG TAG GCA CCT AC | |
| O3.4 | CTC AAG TAG GTG CCT ACT TAC | |
| O4.1 | CTC CAA TAA TTG CTC CAA GC | |
| O4.2 | GCT TGG AGC AAT TAT TGG AG | |
| O5.1 | GCC ACC AGA AGT AGG GTT G | |
| O5.3 | GGG GAT GTT GTC GCA AAT G | |
| O5.4 | CAT ACT CCG ACT CAG GCC | |
| PsaA.1 | CGT TCC GAT TGG GCA AGA C | |
| PsaA.2 | GCA CTT GGA ACA CCA TAG |