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. 2004 Aug;72(8):4344–4350. doi: 10.1128/IAI.72.8.4344-4350.2004

FIG. 2.

FIG. 2.

Binding of AAT to EspB and EspD in an overlay assay. (A) Supernatant proteins of EPEC strains 3431 and E2348/69 grown under conditions inducing the secretion of Esp's were separated by SDS-PAGE and blotted to nitrocellulose, which was subsequently incubated with AAT. Binding of AAT to EspB and EspD was detected with anti-AAT antiserum. (B) Additionally, equivalent amounts of protein were separated by SDS-PAGE and stained with Coomassie brilliant blue to determine the relative amounts of proteins in the supernatants.