CAII and CAIV protein detection changes with puberty in the male reproductive tract.
A, CAII protein detection by immunohistochemical staining appears with the onset of puberty (5 weeks) in elongated spermatids, single epithelial cells, and the stereocilia network of the caput, corpus, and cauda epididymidis. B, CAIV immunoreactivity is localized in 3-week-old mice only in the stereocilia network of the corpus epididymidis but not detectable in the testis, caput, and cauda epididymidis. In tissues from pubescent mice, CAIV is present in the corpus and cauda epididymidis (Scale bars = 25 μm).