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. 2015 Oct 19;290(49):29281–29289. doi: 10.1074/jbc.M115.696005

FIGURE 5.

FIGURE 5.

The transmembrane part of TatE is in close proximity to TatC. INV containing, in addition to the indicated Tat components, the Leu-9Bpa variants of TatE and TatA as well as the Asp-150Bpa variant of TatC were UV irradiated and analyzed by immunoblotting using antibodies against all Tat components (αTatE, αTatA, αTatB, and αTatC). The ∼30 kDa adduct of TatELeu-9Bpa to TatC is marked with an orange square and that of TatCAsp150Bpa to TatE with a blue square. Cross-links between TatALeu-9Bpa and TatC are indicated by magenta squares. They disappear in the presence of TatE (lanes 12 and 14). The TatE-TatA dimer is marked with an orange dot and the TatA-TatE dimer with a magenta dot. Homo-oligomers of TatE and TatA are labeled with orange and magenta stars, respectively. Note complexes of the size of a TatA dimer appearing even when TatA was Bpa-free (e.g. lanes 4 and 8) because of SDS-resistant oligomerization of TatA. The blue diamond indicates the interaction of TatB with position Asp-150 in TatC.