FIGURE 7.

FYCO1 is required for efficient maturation of autophagosomes in a LIR-dependent manner during basal autophagy. A, Western blots of LC3A/B in total extracts from HEK293 WT, FYCO1 KO, and reconstituted KO cells with GFP-FYCO1 WT and LIRmut, respectively. FM, full medium; BafA1, bafilomycin A1. B, relative levels of LC3 II normalized to the actin loading control. The blots were quantified using ImageJ. Error bars denote S.D. from three independent experiments. C, autophagy flux in full medium calculated by subtracting the LC3 II level without bafilomycin A1 from the LC3 II level in the bafilomycin A1-treated samples. Flux for each experiment was displayed as the percentage of the flux in the control HEK293 cell line. *, p < 0.05, **p < 0.01 (one-sample t test). NS, not significant.