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. 2015 Oct 5;290(49):29402–29413. doi: 10.1074/jbc.M115.680199

FIGURE 1.

FIGURE 1.

Ceramides activate the Nlrp3 inflammasome via mitochondrial oxidative stress. A, representative Western blot analysis of IL1β (active p17) protein in supernatants of WT BMDMs that have been primed with LPS for 4 h and stimulated with increasing doses of ceramide (C6) for 6 h. B, Western blot analysis of IL1β protein in BMDMs from WT or Nlrp3−/− mice that have been stimulated with LPS, LPS plus C6 (80 μg/ml) for 6 h, or LPS plus palmitate (400uM) for 24 h. C, Western blot analysis of caspase-1 (active p20) and catalase protein in BMDMs from WT or MCAT transgenic mice that have been treated with LPS alone, LPS plus C6, or LPS plus ATP (5 mm) for 1 h. Two representative blots from individual experiments are shown. D, schematic depicting palmitate entry into oxidative pathway to generate ATP or the nonoxidative pathway causing the de novo synthesis of ceramide. E, Western blot analysis of IL1β protein in the supernatants of WT BMDMs, treated with LPS or LPS plus palmitate, in which some received pretreatment with serine palmitoyltransferase inhibitor, myriocin. The data are representative of two or three individual experiments. Exp., experiment.