FIGURE 3.

Sptlc2 is not required for macrophage polarization or inflammasome activation in vitro. A, iNos, Tnfα, or arg1 gene expression, normalized to gapdh, from CRE⁻ or CRE⁺ BMDMs that have been polarized to M1 (filled bars) or M2 (open bars). B, two Western blots from individual experiments of IL1β protein in supernatants of CRE⁻ or CRE⁺ BMDMs following no treatment, treatment with LPS alone, LPS plus palmitate, LPS plus ATP, or LPS plus C6 (n = 2–3 individual experiments). The error bars represent means ± S.E. Exp., experiment.