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. 2015 Oct 5;290(49):29402–29413. doi: 10.1074/jbc.M115.680199

FIGURE 4.

FIGURE 4.

Myeloid cell-specific Sptlc2 is not required for diet-induced inflammation. A–C, Body weight (BW, A), visceral adipose tissue weight (AT, B), and cells per gram of adipose tissue (gAT, C) after 13 weeks of LFD or HFD in CRE− or CRE+ mice. D, gating strategy to analyze stromavascular fraction (SVF) of adipose tissue. E, representative dot plots of F4/80+CD11b+ cells from adipose tissue of CRE− mice on LFD, CRE− mice on HFD, or CRE+ mice on HFD. F4/80+CD11b+ cells were gated on to analyze CD206 and CD11c expression. F, quantification of the percentage of macrophage and the macrophage subpopulations. G, quantification of the percentage of lymphocytes, CD3+ T cells, and B220+ B cells, in adipose tissue from HFD-mice (n = 9–10 biological replicates; one-way ANOVA or t test as appropriate). *, p < 0.05. The error bars represent means ± S.E.