FIGURE 2.

AUC results of the SMC hinge domains. a–c, equilibrium concentration gradients in sedimentation equilibrium AUC were indicated for hSmc2H-CC30 (a), hSmc4H-CC30 (b), and hSmc2H-CC30/hSmc4H-CC30 (c). d, concentration dependences of 1/M_app (where M_app indicates apparent molecular weight) for each sample. Molecular weight estimates and deduced oligomeric states were summarized in Table 1. e, sedimentation velocity AUC results of hSmc2H-CC30 (solid line), hSmc2H(G/D)-CC30 (dashed line), and a mixture of hSmc2H-CC30 and hSmc4H-CC30 (dotted line) were indicated. Oligomeric states of each protein, which were identified based on the estimated molecular weights, were also shown.