Skip to main content
. 2015 Oct 20;290(49):29461–29477. doi: 10.1074/jbc.M115.670794

FIGURE 6.

FIGURE 6.

The hydrogen uptake ratios of hSmc2H-CC30/hSmc4H-CC30 heterodimer at different time points. The time points correspond to the duration of hydrogen deuterium exchange reaction, which was produced by reducing the pH to 2.5 at different timing from the beginning of the reaction. a, hSmc2H-CC30. b, hSmc4H-CC30. Each horizontal color block indicates an analyzed digested peptide, and each block contains a number of time points. c–f, hSmc2H-CC30 (c), hSmc2H-CC30 under the presence of ssDNA (d), hSmc4H-CC30 (e), and hSmc4H-CC30 under the presence of ssDNA (f). The deuteration level of each peptide at each time point is color-coded. The figures in a–f were generated using all identified peptides that could be used for deuterium exchange analysis. g, commonly identified peptides in hSmc2H-CC30 for hSmc2H-CC30/hSmc4H-CC30 heterodimer in the absence and presence of ssDNA. h, those in hSmc4H-CC30 for hSmc2H-CC30/hSmc4H-CC30 heterodimer.