TABLE 3.
Thermodynamic parameters of ssDNA binding to hSmc2H-CC30/hSMC4H-CC30 heterodimer obtained from the ABB model
The values of Kd and ΔH were calculated by using the best fit value obtained from the global analysis of the triplicate data (supplemental Table S1). The values of ΔG and −TΔS were calculated by using the thermodynamic relationships. A was defined as ssDNA, and B was defined as hSmc2H-CC30/hSMC4H-CC30 heterodimer
| Binding site | Parameters |
|||
|---|---|---|---|---|
| Kd | ΔG | ΔH | −TΔS | |
| nm | kcal mol−1 | kcal mol−1 | kcal mol−1 | |
| Primary | 22.3 | −10.4 | −1.26 | −9.18 |
| Secondary | 2500 | −7.64 | −4.78 | −2.86 |