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. 2015 Oct 21;290(49):29493–29505. doi: 10.1074/jbc.M115.670364

FIGURE 3.

FIGURE 3.

Differentiation of DMRC-iPSCs into hepatocytes. A, overall protocol for hepatic differentiation of hiPSCs. B, transcriptional expression of hepatic genes in DMRC hepatocytes. C, efficiency of hepatic differentiation. Hepatocytes differentiated from hiPSCs were analyzed by flow cytometry for albumin, a hepatic marker. D, expression of the hepatic marker albumin in WT and DMRC hepatocytes. Scale bar, 50 μm. E, albumin secretion in WT and DMRC hepatocytes. F, visualization of glycogen storage by PAS staining co-stained with α1-antitrypsin (AAT). Scale bar, 50 μm. G, decrease of the glycogen level in DMRC hepatocytes. ***, p < 0.001 (n = 3). HEP, hepatocytes; NC, negative control.