FIGURE 3.

Inositol rescues cytoplasmic Ca²⁺ oscillations in LiCl-treated cells. A, oscillations in cytoplasmic [Ca²⁺]_i evoked by LTC₄ are sustained in a LiCl-treated cell when 10 mm inositol is added shortly before stimulation. B, aggregate data comparing the number of oscillations over an 800-s recording period for the conditions shown. Each column is the average of >30 cells from four independent experiments. **, p < 0.01; ****, p < 0.0001; ns, not significant. C, exposure to 10 mm inositol increases the oscillation frequency in a control cell. D and E, inositol fails to rescue oscillations in [Ca²⁺]_i evoked by LTC₄ in a LiCl-treated cell when CRAC channels are blocked with either La³⁺ (D) or BTP2 (E). F, aggregate data are compared for >50 cells/point. Each bin number reflects 200 s of recording. G, cytoplasmic Ca²⁺ oscillations are maintained over 800 s when a cell is challenged with LTC₄ in Ca²⁺-free solution containing 1 mm La³⁺. H, Ca²⁺ oscillations run down in 0 Ca²⁺solution containing 1 mm La³⁺ following pretreatment with 15 mm LiCl, and this cannot be rescued by inositol. I, aggregate data are compared. Cells were stimulated with LTC₄ in 0Ca²⁺ solution containing 1 mm La³⁺. ****, p < 0.0001.