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Both lipin 1 and c-Fos co-localize with the ER. Confocal images of quiescent NIH 3T3 cells that were stimulated for 1 h with 20% FBS, fixed, and immunostained for endogenous c-Fos (a) or lipin 1 (b). c, calreticulin-GFP was used as an ER marker. The co-localization mask in white (obtained with ImageJ “Colocalization Finder” plug-in) shows co-localization of c-Fos (d) or lipin 1 (e) with the ER. f, white mask of pixels where both lipin 1 and c-Fos co-localize with the ER. Bar, 5 μm.
