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Binding of Twist1 to the Fgf10 promoter. A ChIP assay was conducted with a Twist1-specific antibody and nuclear extracts from MDPC23 cells. The PCR analysis identified a specific PCR product of 169 bp corresponding to the region −1101∼1269 nt of the Fgf10 promoter in the input added with Anti-Twist1 antibody (T) but not in the input with normal IgG as a negative control (N). I, the nuclear extracts were used as a PCR-positive control.
