FIGURE 9.
Ameloblastin expression in ameloblasts in Twist2Cre/+;Twist1fl/fl molars. Immunofluorescence staining of the E18.5 control and the E18.5 Twist2Cre/+;Twist1fl/fl molars 3 weeks after kidney capsule transplantation with an antibody against ameloblastin. Stronger ameloblastin immunofluorescent signals (red) were strictly observed in the ameloblasts of the cuspal regions in the control molars (A–C). However, a much weaker intensity of the signals was detected in the Twist2Cre/+;Twist1fl/fl molars (D--F). Nuclei were counterstained with DAPI (blue). Scale bars = 750 μm.