B7-H6 expression in tumor cells correlates to their susceptibility to NK killing.
A and B, HEK293 cells were transduced with lentivirus delivering scramble shRNA (shScr) or shRNA targeting B7-H6 (shB7-H6-1, shB7-H6-2), and B7-H6 expression was then analyzed by real-time PCR (A) and Western blotting (B). C, lysis of HEK293 cells transduced with shScr, shB7-H6-1 or shB7-H6-2 by NK-92 cells was determined in a 4 h cytotoxicity assay. D, lysis of K562, HL60, U937, or HeLa cells transduced with shScr, shB7-H6-1, or shB7-H6-2 by NK-92 cells was determined in a 4 h cytotoxicity assay.