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. 2015 Oct 15;290(50):29964–29973. doi: 10.1074/jbc.M115.674010

FIGURE 7.

FIGURE 7.

Non-lethal heat shock increases B7-H6 expression and makes target cells more sensitive to NK cell cytolysis. A and B, HEK293 cells were exposed to 42 °C for 30 min and then cultured at 37 °C for the indicated times. B7-H6 expression was analyzed by real-time PCR (A), Western blotting (B). HEK293 cells were exposed to 42 °C for 30 min and then cultured at 37 °C for 12 h and B7-H6 expression was analyzed by flow cytometry (C). D, HL60 cells were exposed to 42 °C for 30 min and then cultured at 37 °C for the indicated times. B7-H6 expression was analyzed by Western blotting. E, HL60 cells were exposed to 42 °C for 30 min and then cultured at 37 °C for 12 h. B7-H6 expression on cell surface was analyzed by flow cytometry. F, HEK293 cells were incubated at 42 °C for 30 min, cultured at 37 °C for 11 h, and then subjected to NK-92-mediated lysis. G, HEK293/shScr, HEK293/shB7-H6-1, and HEK293/shB7-H6-2 were exposed to 42 °C for 30 min or not, then cultured at 37 °C for 11 h and subjected to NK-92-mediated lysis.