FIGURE 2.

CD22 is unmasked on human GC B-cells compared with naive and memory B-cells. Flow cytometry staining of naive (CD27⁻CD38^low), GC (CD27⁺CD38⁺), and memory (CD27⁺CD38⁻) human tonsillar CD19⁺ B-cells with KN343 (A), human CD22-Fc (B), anti-human CD22 (C), and fluorescent liposomes (D) displaying no ligand (naked) or a selective human CD22 ligand (6′^BPCNeu5Ac; targeted). Quantitated ΔMFI (mean fluorescence intensity) values represent background-subtracted values and represent the average and standard error of three replicates and is representative of three independent experiments. *, p < 0.05; N, naive; G, germinal center; M, memory. Statistical analysis was performed by Student's t test.