FIGURE 4.

Nucleotide incorporation by Polη opposite an undamaged A and 1-MeA. Polη (0.25 nm) was incubated with DNA (10 nm) and with 25 μm of a single nucleotide (A, T, G, or C) or 25 μm of each of four dNTPs (N) for 10 min at 37 °C. DNA substrate is shown above the gel; the asterisk indicates the site of either an A or a 1-MeA residue.