TABLE 2.
Primers used in this study
F, forward; R, reverse. The underlined nucleotides designate respective restriction sites.
| Primer name | Sequence (5′–3′) | Purpose |
|---|---|---|
| SDP13 F | ATCTTTCAGGATTAAAAAAT | hcaE knock-out |
| SDP14 R | CTCTAGTGAAACTTGCGCAC | |
| SDP15 F | GAACCGAGTCGTGAGGTACT | mhpA knock-out |
| SDP16 R | AGTGAAGATAAGCGTGCATA | |
| SDP17 F | ATTTTGTTGTTAAAAACATGTAA | mhpR knock-out |
| SDP18 R | CCACCAGAATAGCCTGCGAT | |
| SDP19 F | GCTATCGAGCCACAGGACTG | paaA knock-out |
| SDP20 R | ATTACTCATTTTGAATCTCC | |
| SDP21 F | AACATCTGACGAGGTTAATA | maoA knock-out |
| SDP22 R | CGTTTTTTTGTCTGAAACAA | |
| SDP31 F | AGTCGAACGGTAACAGGAAGA | 16S rRNA qPCR |
| SDP32 R | GCAATATTCCCCACTGCTG | |
| SDP33 F | GAAAGAGCACGATCGCGTGG | aceB qPCR |
| SDP34 R | TTGCTCGGAATAAACGCCGC | |
| SDP35 F | GAAAGAGCACGATCGCGTGG | aceK qPCR |
| SDP36 R | GCAAGCTGGCGAATAGCGTT | |
| SDP37 F | TAAGGACACGTTGCAGGCCA | acnA qPCR |
| SDP38 R | GGCGGTAGGCAATTTCACGG | |
| SDP39 F | TGGTTCCCGTGAAGCTCTGG | eno qPCR |
| SDP40 R | TGCAGCAGCTTTGGCGTTAG | |
| SDP41 F | CAATTCGCGGGGTTGTTCGT | pfkA qPCR |
| SDP42 R | GGTTTTCGATAGCCACGGCG | |
| SDP43 F | GCAGAAATGCTGGCGCGTAT | fadA qPCR |
| SDP44 R | ATGTGCCCGCCGTTACCATA | |
| SDP45 F | ACCGCTAATGAAGTGGCCGA | fadR qPCR |
| SDP46 R | CTGCACAACGCCGACAGTTT | |
| SDP47 F | TGCTCGGTATGGCACCGAAT | glcB qPCR |
| SDP48 R | GGCTTTGATCCAAGGCGTCG | |
| SDP49 F | TACTGAAGGTCGGTCAGCCG | glcC qPCR |
| SDP50 R | CCTCCAGTAATGCGCGAACG | |
| SDP51 F | CGAAGGCGGCACCGAAATTG | hcaE qPCR |
| SDP52 R | CGGGCGGTTTGTCCATCACC | |
| SDP53 F | CAGACAGCCAGACACCTTGA | hcaR qPCR |
| SDP54 R | CGGATCGGTACTGACGAAA | |
| SDP55 F | CCCGGTTGGGCTGATGATGG | mhpA qPCR |
| SDP56 R | CGGCGTAGTGTGCGGCAGAA | |
| SDP57 F | GCGGTACGGCGATAGAGGCG | mhpR qPCR |
| SDP58 R | ACCTGGCTGGCCTTTTGCCC | |
| SDP59 F | CCACCGACGAATTACGCAGC | prpB qPCR |
| SDP60 R | TTCACCTGGCTACGGGCAAA | |
| SDP61 F | TATTTTGCCCGCCACGATGC | prpR qPCR |
| SDP62 R | GCGGCATTTCGCCAATCTCA | |
| Orf306F2 | GGCTGCCATCATCCCATGGGAACACCCGCTA | Amplification of orf306 as NcoI-BamHI fragment to be cloned in pET15b |
| Orf306R3 | CGCGGATCCCTATTCCCCGTCAAGATAC | |
| Orf306F5 | GGCTGCCATCATCGGATCCGAACACCCGCTA | Amplification of orf306 as BamHI fragment using |
| Orf306R3 as reverse primer to be cloned in pGEX4T1 | ||
| HcaRFP1 | AGGAATTCAAGCTCCAGTTGGTAA | Amplification of hcaR promoter to be cloned in pMP220 |
| HcaRRP1 | ACCTGCAGACGGGTAAAGTTCAGT | |
| HcapET23bFP1 | ATACGCATATGACCACACCCTCAGATTTGAA | Amplification of hca operon as NdeI-SalI fragment to be cloned in pET23b |
| HcapET23bRP1 | ACTACGTCGACCAGTGATTTAAGCGCGATGTT | |
| MhpRF | CCGAGCTGCAGATTAATTGACATTTCTATA | Amplification of mhpR promoter to be cloned in pMP220 |
| MhpRR | CCGGAGAATTCTTCAGTACCTCACGAC |